The service for 14C radiolabeling provides accurate isotope labeling of compounds for thorough examination, facilitating studies on pharmacokinetics, metabolism, and distribution. Alfa Cytology has the necessary technology to perform 14C-Radiolabeling and produce preclinical research of the highest quality and greatest accuracy.
14C isotopes refer to carbon 14, which is a radioactive isotope of carbon and is used in various scientific studies, particularly in the fields of radiolabeling, metabolic studies, and pharmacokinetics. 14C has a half-life of 5730 years and hence is stable enough for long analysis but decays enough to successfully track compounds in biological systems. 14C is used for labeling various small molecules, peptides, and biologics, which helps in studying the absorption, distribution, metabolism, and excretion (ADME), and pharmacokinetics of the compounds for preclinical studies.
14C radiolabeling is one of the most important and fundamental techniques used in the preparation of radiopharmaceuticals, which assists in tracking the distribution of the drugs, the metabolism, and the pharmacokinetics. With the addition of the radioactive isotopes of carbon to a compound, a researcher has the ability to study the pharmacokinetics and biochemistry of the compound in a living system without altering the chemical structure of the compound. This is important in developing a drug to understand the interactions of a drug with a receptor.
Fig 1. Carbon-14 radiolabeling: a traceless tool for tracking organic molecules, supporting drug development and the agrochemical industry. (BABIN V, et al., 2022)
High Sensitivity
14C has an extremely high detection sensitivity, reaching a maximum specific activity of 2.3 MBq/mmol (1.2 × 1011 dpm/mmol) and allowing detection of extremely low levels (ppt) of this radioisotope. In contrast, high-resolution mass spectrometry can only achieve sensitivity at the ppb level.
Reliable Experimental Results
Isotope-labeled tracer technology yields more reliable results than control experiments, as it is independent of matrix effects, background interference, and other analytical and/or interferences. The half-life of 14C is 5,370 years, which means that the correction for radioactive half-life is eliminated in experimental data.
Minimal Isotope Effect
When 14C replaces 12C atoms, the impact will be minimal on the chemical reactivity for the whole molecule. Meanwhile, the substitutive isotopism of hydrogen will considerably alter the physical and chemical properties, which can hinder the signs of the metabolism and the distributions of biological and environmental compounds.
High Labeling Stability
While performing labeling reactions, 3H tends to undergo exchange reactions with stable hydrogen in water, which leads to a loss of labeling. In contrast to this, carbon labeling positions are often on the molecular backbone, which ensures that the 14C labeling remains stable.
Alfa Cytology provides services with unrivaled quality due to advanced know-how and state-of-the-art capabilities in 14C-radiolabeling for tracking compounds in various lines of research. Our services support highly accurate and dependable studies in pharmacokinetics, metabolism, and environmental studies.
Alfa Cytology offers custom 14C conjugation services, providing tailored solutions for the precise radiolabeling of compounds, including small molecules, antibodies, and other biologics, to meet specific research needs.
Built upon a foundation of versatile and well-established synthetic methodologies, our capabilities are structured to handle a wide array of complex molecular targets. For more efficient and streamlined processes, we have structured our expertise in synthetic chemistry into three primary schemes that allow for specialization:
Systematically collect a complete set of biological samples at various time points post-dose, including:
Carbon-14 (14C) radiolabeling is a versatile and powerful method with applications across various scientific disciplines. Its unique properties make it invaluable for tracing and quantifying carbon-containing compounds in complex systems.
Pharmaceutical and Biochemical Research
The practical application and trustworthiness of our labeling expertise and technology can be seen in our Helicobacter pylori diagnosis products. This product can accurately collect and quantify 14C-labeled CO2, which confirms H. pylori infection with great sensitivity and specificity.
| Product Name | Description |
|---|---|
| Helicobacter pylori Urea Breath Test Kits | The principle that mammalian cells do not have urease, and H. pylori do have it in abundance, is the basis for our non-invasive breath test platform. After the oral administration of our 14C-labeled urea, any H. pylori bacteria present will hydrolyze it and release 14C-labeled carbon dioxide. This gas moves into the bloodstream and is exhaled. |
Having a stock of key carbon-14 building blocks helps us expedite our research timelines. Our stock includes key precursor materials such as Barium Carbonate [14C], Urea [14C], and Aniline [14C(U)] Hydrochloride. Additionally, we have some pre-synthesized, complex, labeled compounds like some amino acids, sugars, lipids, and sterols, which help us custom-synthesize projects.
| Types | Product Name | |
|---|---|---|
| Radiolabeled Precursors | Aniline [14C(U)] hydrochloride Acetic acid (salt), [1-14C] Barium carbonate [14C] Benzene, [14C(U)] Benzyl alcohol, [7-14C] CuCN[14C] Methanol[14C] Methyl iodide, [14C] NaCN[14C] Propionic acid (salt), [1-14C] Pyruvic acid (salt), [1-14C] |
Acetyl chloride, [1-14C] Bromoacetic acid, [1-14C] Benzaldehyde, [carbonyl-14C] Benzoic acid, [7-14C] Carbon dioxide[14C] Ethanol, [1-14C] Formic acid (salt), [14C] Glycine, [1-14C] Urea[14C] Thiourea, [14C] And more |
| Radiolabeled Amino Acids | Arginine L-[14C(U)] Leucine, L-[14C(U)] Glycine, [14C(U)] Phenylalanine-L-[14C(U)] |
Alanine, L-[14C(U)] Glutamic Acid, L-[14C(U)] Isoleucine, L-[14C(U)] Lysine, L-[14C(U)] |
| Radiolabeled Sugars | GLUCOSE, D-[14C(U)] GLUCOSE, D-[6-14C] XYLOSE, D-[14C(U)] SUCROSE, [14C(U)] |
GLUCOSE, D-[1-14C] DEOXY-D-GLUCCOSE, 2-[1-14C] GALACTOSE, D-[1-14C] And more |
| Radiolabeled Lipids and Sterols | GLYCEROL, [14C(U)] CHOLESTEROL, [4-14C] |
OLEIC ACID, [1-14C] PALMITIC ACID, [1-14C] |
| …… | …… | …… |
At Alfa Cytology, we are fully committed to the development and innovation of 14C radiolabeled services. We harness our considerable technical skills and state-of-the-art research to professionally facilitate progress in the. If you need any further information about our services, please get in touch with us.
Reference
For research use only. Not intended for any clinical use.
Alfa Cytology, a provider of RDC preclinical services, specializes in RDC design, conjugation, and analysis. Supported by a rich library of radionuclides, chelators, and linkers, we excel in targeting molecule design. We offer customized RDC services tailored to diverse requirements-your demand is our mission.
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